|ORIGINAL RESEARCH REPORT
|Year : 2021 | Volume
| Issue : 2 | Page : 98-102
Gastrointestinal stromal tumor experience in a surgical oncological unit in sub-Saharan Africa: A retrospective analysis
Omobolaji O Ayandipo1, Gabriel O Ogun2, Oluwasanmi A Ajagbe3, Omolade O Adegoke2, Olalekan J Adepoju4, Adam Rahman4, Chioma M Ajuyah3, Akintunde T Orunmuyi5, Olayiwola B Shittu1
1 Department of Surgery, College of Medicine, University of Ibadan, Ibadan, Nigeria
2 Department of Histopathology, College of Medicine, University of Ibadan, Ibadan, Nigeria
3 Department of Surgery, University College Hospital, Ibadan, Nigeria
4 Department of Histopathology, University College Hospital, Ibadan, Nigeria
5 Department of Radiation Oncology, College of Medicine, Ibadan, Nigeria
|Date of Submission||10-Jul-2020|
|Date of Acceptance||09-Sep-2020|
|Date of Web Publication||24-Apr-2021|
Dr. Akintunde T Orunmuyi
Department of Radiation Oncology, College of Medicine, University of Ibadan/University College Hospital, Ibadan
Source of Support: None, Conflict of Interest: None
Background: Gastrointestinal stromal tumors (GISTs) are characterized by specific immunohistochemical and genetic features. This study investigated the clinicopathologic features and surgical outcomes of 54 patients with GIST in a major referral hospital in sub-Sahara Africa. Methods: This retrospective cross-sectional single-center study describes 54 patients who underwent surgery for GIST between 1999 and 2019 in a Nigerian Tertiary Hospital. All cases were confirmed by immunohistochemistry and were analyzed for clinical characteristics, clinical management, and histopathologic data. The risk of recurrence was determined using the Fletcher National Institutes of Health criteria. Results: The mean age of the patients was 57.6 years ± 15.3 (11–81 years), and most patients (67%) underwent abdominal computed tomography scans preoperatively. The most common presentation was an abdominal mass (48%). The stomach was the most common site of GIST (37%). The mean tumor size was 8.9 cm (5–18 cm), and tumor morphology showed spindle cell type (75.9%), mixed spindled-epithelioid (20.3%), and epithelioid (3.7%) cell types. Overall, 51% had a high risk for recurrence. Conclusion: The clinicopathologic features of GIST in this study are consistent with reports in the literature. Slight variations in risk profile may be due to late presentation in our settings.
Keywords: Africa, gastrointestinal stromal tumor, immunohistochemistry, risk, surgery
|How to cite this article:|
Ayandipo OO, Ogun GO, Ajagbe OA, Adegoke OO, Adepoju OJ, Rahman A, Ajuyah CM, Orunmuyi AT, Shittu OB. Gastrointestinal stromal tumor experience in a surgical oncological unit in sub-Saharan Africa: A retrospective analysis. J Clin Sci 2021;18:98-102
|How to cite this URL:|
Ayandipo OO, Ogun GO, Ajagbe OA, Adegoke OO, Adepoju OJ, Rahman A, Ajuyah CM, Orunmuyi AT, Shittu OB. Gastrointestinal stromal tumor experience in a surgical oncological unit in sub-Saharan Africa: A retrospective analysis. J Clin Sci [serial online] 2021 [cited 2021 May 17];18:98-102. Available from: https://www.jcsjournal.org/text.asp?2021/18/2/98/314452
| Introduction|| |
Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors that occur at any site of the gastrointestinal (GI) tract. They may occur without a direct connection to the GI tract and are appropriately termed extra-gastrointestinal GIST (E-GIST), but are objectively identified by immunohistochemistry (IHC). GIST and the description of its epidemiology in sub-Sahara Africa are scanty. This retrospective study evaluates the clinicopathologic characteristics of GIST at a major surgical oncology unit in Ibadan, South-Western Nigeria.
GISTs can develop at any age but are mostly found in people over the age of 50 years and predominantly in the sixth decade. Population-based epidemiological reports show an equal gender distribution among patients with GIST.,,,,,,, However, the incidence of GISTs shows a wide variation across geographical regions.,,, The most common sites where GISTs occur are the stomach and small intestine. While small-sized GISTs tend to be asymptomatic and discovered incidentally, 80% of GISTs are symptomatic, presenting with gastrointestinal bleeding, abdominal pain, or abdominal masses.
The gain of function mutations, which appear to occur in several oncogenes but mainly in the tyrosine-protein kinase (c-KIT) receptors and platelet-derived growth factor receptor alpha (PDGFRA), objectively identify GISTs. In addition to sporadic mutations expressed in GIST, familial GIST may also occur as an additional component of specific syndromes, including neurofibromatosis type 1 (NF1) and Carney dyad. The presence of multiple GIST at the time of diagnosis may depict familial GIST, which are inherited germline mutation of the c-KIT or PDGFRA genes. However, some GISTs do not express c-KIT ab-initio (wild type GIST), while others may become refractory to first-line selective c-KIT inhibitors following secondary c-KIT mutations. Thus, other immunohistochemical evaluations for GIST include succinate dehydrogenase (SDH) complex deficiency, smooth muscle actin, S-100, and vimentin.,
Management of gastrointestinal stromal tumor
Curative surgical resection is the mainstay of treatment, and up to 60% of patients are cured with surgery alone. However, the tyrosine kinase inhibitors (TKIs) are of great significance in the management of GISTs. Presurgical biopsy, preferably endoscopic, enhances the surgical resection of large GISTs and identification of patients who are more likely to develop recurrence.,, Clinical prognostic and predictive factors are commonly used to estimate the risk of recurrence and predict the potential benefit of TKI therapy. The tumor size, anatomical location, and mitotic count are the main prognostic factors for GIST. Contrast-enhanced computed tomography (CT) imaging is the standard for evaluation of tumor size, location, and metastases and is of immense value for guiding biopsy. TKI therapy is recommended for patients with high risk, metastatic or unresectable GIST and is associated with improved prognosis and treatment outcomes. However, the development of secondary mutations while on TKI therapy is not uncommon., Consequently, positron emission tomography (PET) imaging plays a valuable role in early evaluation and confirmation of treatment response to TKI and encourages curative surgery for excision of non-responsive metastatic GIST.
| Methods|| |
This study was conducted in compliance with the guidelines of the Helsinki declaration on biomedical research on human subjects., The medical records of all cases of GIST confirmed by IHC using CD 117 or DOG 1, between 1999 and 2019, were identified from the database of the Departments of Pathology and Surgery of a tertiary center in Southwest Nigeria. Anatomical origin, histologic cell type, and IHC were collated from the final pathology report, while a description of the tumor size, subcutaneous fat layer, and tumor appearance was collated from surgery notes. Demographic data and clinical presentation were collated from the case files. All cases managed had their records archived, retrieved, and analyzed. A high mitotic rate was taken as >5/50 and <5/50 high power field (HPF) as low. The risk of recurrence was determined using the Fletcher classification based on tumor size and mitotic count in 50 HPFs. Briefly: Very low risk if tumor size <2 cm and mitotic count <5/50 HPF; low risk if tumor size between 2 and 5 cm and <5/50 HPF; intermediate risk if size <5 cm and 6–10/50 HPF or size 5–10 cm and <5/50 HPF; and high risk for >5 cm and >5/50 HPF or any size >10 cm or mitotic count >10. Descriptive statistics estimated the means and standard deviations for continuous variables, while percentages were computed for categorical variables.
| Results|| |
Overall, 54 cases of histologically confirmed GIST presented during the study period and were analyzed.
There were 26 (48%) men and 28 (52%) women demonstrating a male-to-female gender ratio of 1:1.07. The mean age was 57.6 ± 15.3 years (range 11–81 years), and nearly two-thirds were aged >50 years [Table 1].
|Table 1: Clinco-epidemiological characteristics: age, gender, clinical presentation|
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The most common presentation was an abdominal mass (26 cases, 48%), followed by abdominal pain (15 cases, 27.7%) and gastrointestinal bleeding (7 cases, 12.9%). In 6 (11.1%) patients, GIST was an incidental finding. Overall, 36 (67%) patients underwent CT scans prior to surgery. Tumor appearance was a classical fleshy exophytic mass in all cases and there was no loss of the submucosa layer reported. The stomach was the most common site of GIST (20 cases, 37%) followed by the omentum, while the esophagus was the least common site [Table 2]. Although tumor sizes ranged from 5 cm to 18 cm with a mean size of 8.9 cm, most of the tumors (62.9%) were between 5 and 10 cm. Microscopic examination revealed complete tumor resection (R0) in 28 (51.8%) patients, microscopically positive margins (R1) in 13 (24.0%) patients and macroscopically positive margins in (R2) 10 (18.5%) patients [Table 3]. Three patients (5.5%) had unresectable masses which were biopsied for pathologic and immunohistochemical analysis.
|Table 2: Surgical characteristics: gross appearance, anatomical location|
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|Table 3: Pathologic characteristics: resection margins, morphology, mitotic activity, mutational analysis|
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The spindle cell tumor was the most common histologic type (75.9%), followed by mixed spindled and epithelioid type in 11 cases (20.3%) and epithelioid type in 2 cases (3.7%). Overall, mitotic counts ranged from 1 to 60 per HPF, with 32 (59%) patients showing high mitoses and low mitoses in the remainder. Risk stratification by National Institutes of HealthNIH-Fletcher's criteria placed 28 (51.8%) patients and 1 patient (1.8%) into high- and low-risk categories, respectively. The remainder were the intermediate-risk category. IHC findings using CD117 and DOG1 was positive in 34 and 20 patients, respectively [Table 3].
| Discussion|| |
This study of 54 patients with GIST managed over a 20 years period is the largest in this part of the world. Following a systematic review by Ogun et al., this study represents the largest single-center study from Africa. The demography of GIST patients in this study is consistent with other reports that indicate a male-to-female gender ratio close to 1:1. Søreide et al. reported a fairly consistent equal distribution between males and females. The mean age of 57.6 ± 15.3 years in our cohort compares fairly with larger study populations by Lv et al. in which the mean was 60 ± 12.9 years and 61.18 ± 14.13 years by Ud Din et al. The age range is also consistent with the reported range of 10–100 years from a systematic review by Søreide et al. However, in patients younger than 50 years, GIST is significantly associated with a favorable outcome. Our study shows the stomach as the most common site, as reported fairly consistently reported between studies.,,,,,,,,,, The lower occurrence of esophageal and colorectal GISTs in this study varies with other studies. This is likely due to the rare occurrence and possibly underreported incidence of GISTs generally. Overall, the colorectal, esophagus, and extra-gastrointestinal sites including, the mesentery and spleen, were few and in keeping with other studies that reported <10% of those with GIST in these locations.,,
The average size of tumors in this study was 8.9 cm and size ranged from 5 cm to 18 cm. Various studies have reported sizes ranging from a few millimeters to >20 cm for small intestinal, and a few millimeters to >40 cm for gastric GISTs. In a large review of gastric GISTs, the mean size for gastric GISTs was 6 cm. In two separate studies, from Iceland and China, the mean tumor size was 4.6 cm and 7.02 cm, respectively., Consequently, the majority (51%) of our patients were classified as high-risk for recurrence. The larger size of tumors in our study may be contribute to the high mitosis rates observed in majority (59%) of our patients. Late presentation among patients in low-income settings and the hospital-based nature of this study may explain the variation with studies reported in the literature, which are mostly population-based studies from the West. Risk classification may contribute toward identification of patients who will require targeted therapy postoperatively. Patients at high risk of recurrence were referred to a regional center for free drug assistance in a program that is still ongoing. The retrospective nature of this study precludes further analysis of recurrence rates in this cohort.
Symptoms in our cases were variable; the most common were vague abdominal pain, abdominal mass, bleeding per rectum, hematemesis, etc., Grossly, majority of our gastric tumors were exophytic submucosal or intramural, pedunculated masses, nodular bulging masses. Some protruded into the gastric lumen. Similarly, gross appearances have been described by various authors.
On histological examination, almost 80% of our cases showed spindle cell morphology, while cases with epithelioid morphology comprised barely 5%, although various international studies have reported the epithelioid type to comprise between 20% and 25%, with mixed tumors comprising the remaining 5%–10% cases.,
Immunohistochemically, CD117 was strongly positive in over 60%. DOG1 showed diffuse strong positivity in over 30%. Although DOG1 is found to be more sensitive and specific as a GIST marker, it would be put to more use in our future publications. Most studies record as high as 90% and 80% positivity for CD117 and DOG1, respectively, the fact that immunochemical analysis was done in retrospect on archival samples collected in the early part of the study period would explain the low values as protein/receptor degradation is inevitable over time. Moreover, DOG1 analysis came into existence only in the past 7 years.
Although different imaging techniques, such as 18 fluorodeoxyglucose (FDG) PET, magnetic resonance imaging, and ultrasonography (US), show utility for diagnostic imaging of GIST, CT is currently the modality of choice. Whereas FDG PET is highly sensitive in the detection of GISTs, its access is limited worldwide. Thus, none of our patients had it. Although access to CT in low resource settings is relative, preoperative diagnosis was complimented with abdominal CT in most of our patients. Before routine availability of CT in our center, the absence of cancer cachexia in a patient presenting with an abdominal mass has been a useful “clinical marker” to suspect GIST, as reported by Ekeblad et al. However, the definitive diagnosis of GIST relies on appropriate sample preservation after surgery for gene analysis and IHC. Therefore, understanding the clinical course and pathology of GIST including the subtle differences that distinguish it from carcinomas is vital in our low resource centres. The well-known symptomatology and clinical profiling at disease presentation should prompt a high index of suspicion. This is important for anticipating the added logistics of fresh frozen sections for intraoperative assessment of negative tumor margins.
While surgical resection remains the mainstay of treatment, meticulous dissection is employed to prevent intraoperative rupture of the tumors and avoid injuries to contiguous structures. Complete resection was possible in 30 (55.3%) of our patients according to the intention to treat principle. This is consistent with the reported spectrum of 48%–89%. Achieving microscopically negative resection margins (R0) however, may not deter recurrence while whole organ removal (e.g., total gastrectomy), is usually not required if a negative resection margin is guaranteed. Although lymphatic metastasis is an extremely rare event in GIST, a “radical” approach, including a lymphadenectomy for metastatic gist, appears to be the consensus. In the rare SDH-deficient GIST, which is present in Carney-Stratakis syndrome, enlarged lymph nodes should be removed.
It is important to resect the tumor with the capsule intact otherwise, seeding on the visceral or somatic peritoneum will occur, leading to recurrence., For GIST in the stomach, the size of the tumor, location, and its proximity to adjacent intra-abdominal viscera may influence the surgical approach undertaken and the extent of stomach resected. In nongastric GIST, en-bloc resection of adjacent organs might be necessary to obtain a clear margin, and care is required to avoid rupture, which may compromise oncological outcomes. In up to 10% of abdominal GISTS, the risk of rupture during surgery is substantial due to extensive neovascularization and the friable nature of the tumor. Rupture into the abdominal cavity has catastrophic sequelae as it almost inevitably leads to peritoneal sarcomatosis.
| Conclusion|| |
GISTs are rare tumors. Although most of our patients had larger tumors and fall into the high-risk category, the clinical epidemiology of GIST in this study is similar to global reports. Surgery remains the mainstay of management, and a high index of suspicion preoperatively presents an opportunity to anticipate the unique diagnostic and treatment features of GIST. Slight variations in risk profile in this study may be related to late presentation in our settings.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Deshaies I, Cherenfant J, Gusani NJ, Jiang Y, Harvey HA, Kimchi ET, et al
. Gastrointestinal stromal tumor (GIST) recurrence following surgery: Review of the clinical utility of imatinib treatment. Ther Clin Risk Manag 2010;6:453-8.
Alkhatib L, Albtoush O, Bataineh N, Gharaibeh K, Matalka I, Tokuda Y. Extragastrointestinal Stromal Tumor (EGIST) in the abdominal wall: Case report and literature review. Int J Surg Case Rep 2011;2:253-5.
DeMatteo RP, Lewis JJ, Leung D, Mudan SS, Woodruff JM, Brennan MF. Two hundred gastrointestinal stromal tumors: Recurrence patterns and prognostic factors for survival. Ann Surg 2000;231:51-8.
Fletcher CD, Berman JJ, Corless C, Gorstein F, Lasota J, Longley BJ, et al
. Diagnosis of gastrointestinal stromal tumors: A consensus approach. Hum Pathol 2002;33:459-65.
Tryggvason G, Gíslason HG, Magnússon MK, Jónasson JG. Gastrointestinal stromal tumors in Iceland, 1990-2003: The icelandic GIST study, a population-based incidence and pathologic risk stratification study. Int J Cancer 2005;117:289-93.
Dematteo RP, Gold JS, Saran L, Gönen M, Liau KH, Maki RG, et al
. Tumor mitotic rate, size, and location independently predict recurrence after resection of primary gastrointestinal stromal tumor (GIST). Cancer 2008;112:608-15.
Kalkmann J, Zeile M, Antoch G, Berger F, Diederich S, Dinter D, et al
. Consensus report on the radiological management of patients with gastrointestinal stromal tumours (GIST): Recommendations of the German GIST Imaging Working Group. Cancer Imaging 2012;12:126-35.
Lv M, Wu C, Zheng Y, Zhao N. Incidence and survival analysis of gastrointestinal stromal tumors in shanghai: A population-based study from 2001 to 2010. Gastroenterol Res Pract 2014;2014:834236. 6 pages.
Kramer K, Knippschild U, Mayer B, Bögelspacher K, Spatz H, Henne-Bruns D, et al
. Impact of age and gender on tumor related prognosis in gastrointestinal stromal tumors (GIST). BMC Cancer 2015;15:57.
Søreide K, Sandvik OM, Søreide JA, Giljaca V, Jureckova A, Bulusu VR. Global epidemiology of gastrointestinal stromal tumours (GIST): A systematic review of population-based cohort studies. Cancer Epidemiol 2016;40:39-46.
Patel N, Benipal B. Incidence of gastrointestinal stromal tumors in the United States from 2001-2015: A United States cancer statistics analysis of 50 states. Cureus 2019;11:e4120.
Martin-Broto J, Martinez-Marín V, Serrano C, Hindi N, López-Guerrero JA, Ramos-Asensio R, et al
. Gastrointestinal stromal tumors (GISTs): SEAP–SEOM consensus on pathologic and molecular diagnosis. Clin Transl Oncol 2017;19:1-10.
Postow MA, Robson ME. Inherited gastrointestinal stromal tumor syndromes: Mutations, clinical features, and therapeutic implications. Clin Sarcoma Res 2012;2:16.
Belinsky MG, Cai KQ, Zhou Y, Luo B, Pei J, Rink L, et al
. Succinate dehydrogenase deficiency in a PDGFRA mutated GIST. BMC Cancer 2017;17:512.
Pantaleo MA, Astolfi A, Urbini M, Nannini M, Paterini P, Indio V, et al
. Analysis of all subunits, SDHA, SDHB, SDHC, SDHD, of the succinate dehydrogenase complex in KIT/PDGFRA wild-type GIST. Eur J Hum Genet 2014;22:32-9.
Roggin KK, Posner MC. Modern treatment of gastric gastrointestinal stromal tumors. World J Gastroenterol 2012;18:6720-8.
Zhao X, Yue C. Gastrointestinal stromal tumor. J Gastrointest Oncol 2012;3:189-208.
Dimitrakopoulou-Strauss A, Ronellenfitsch U, Cheng C, Pan L, Sachpekidis C, Hohenberger P, et al
. Imaging therapy response of gastrointestinal stromal tumors (GIST) with FDG PET, CT and MRI: A systematic review. Clin Transl Imaging 2017;5:183-97.
Williams JR. The Declaration of helsinki and public health. Bull World Health Organ 2008;86:650-2.
Li SJ, Wu YY, Li W, Wang SJ, Fan YM. Ultrastructural observation in a case of mucinous nevus. J Dtsch Dermatol Ges 2018;16:778-80.
Kiśluk J, Zińczuk J, Kemona A, Guzińska-Ustymowicz K, Żurawska J, Kędra B. Expression of CD117, DOG-1, and IGF-1R in gastrointestinal stromal tumours-an analysis of 70 cases from 2004 to 2010. Prz Gastroenterol 2016;11:115-22.
Ogun GO, Adegoke OO, Rahman A, Egbo OH. Gastrointestinal Stromal Tumours (GIST): A Review of Cases from Nigeria. J Gastrointest Cancer 2020;51:729-37.
Ud Din N, Ahmad Z, Arshad H, Idrees R, Kayani N. Gastrointestinal stromal tumors: A clinicopathologic and risk stratification study of 255 cases from Pakistan and review of literature. Asian Pac J Cancer Prev 2015;16:4873-80.
Jumniensuk C, Charoenpitakchai M. Gastrointestinal stromal tumor: Clinicopathological characteristics and pathologic prognostic analysis. World J Surg Oncol 2018;16:231.
Sawaki A. Rare gastrointestinal stromal tumors (GIST): Omentum and retroperitoneum. Trans Gastroenterol Hepatol 2017;4:12-5.
Laroia ST, Yadav T, Rastogi A, Sarin S. Malignant retroperitoneal extra-gastrointestinal stromal tumor: A unique entity. World J Oncol 2016;7:45-50.
Matthews BD, Joels CS, Kercher KW, Heniford BT. Gastrointestinal stromal tumors of the stomach. Minerva Chir 2004;59:219-31.
Rosai J. GIST: An update. Int J Surg Pathol 2003;11:177-86.
Coindre JM, Émile JF, Monges G, Ranchère-Vince D, Scoazec JY. Gastrointestinal stromal tumors: definition, histological, immunohistochemical, and molecular features, and diagnostic strategy. Ann Pathol 2005;25:358-85.
Hong X, Choi H, Loyer EM, Benjamin RS, Trent JC, Charnsangavej C. Gastrointestinal stromal tumor: Role of CT in diagnosis and in response evaluation and surveillance after treatment with imatinib. Radiographics 2006;26:481-95.
Ekeblad S, Nilsson B, Lejonklou MH, Johansson T, Stålberg P, Nilsson O, et al
. Gastrointestinal stromal tumors express the orexigen ghrelin. Endocr Relat Cancer 2006;13:963-70.
Ronellenfitsch U, Hohenberger P. Surgery for gastrointestinal stromal tumors: State of the art of laparoscopic resection and surgery for M1 tumors. Visc Med 2018;34:367-74.
Afuwape OO, Irabor DO, Ladipo JK. Gastrointestinal stromal tumour in Ibadan, Nigeria: A case report and review of current treatment. Afr Health Sci 2011;11:134-8.
Madhavan A, Phillips AW, Donohoe CL, Willows RJ, Immanuel A, Verril M, et al
. Surgical management of gastric gastrointestinal stromal tumours: Comparison of outcomes for local and radical resection. Gastroenterol Res Pract 2018;2018:2140253. 7 pages.
Arifi S, Belbaraka R, Rahhali R, Ismaili N. Treatment of adult soft tissue sarcomas: An overview. Rare Cancers Ther 2015;3:69-87.
[Table 1], [Table 2], [Table 3]